Genomic plasticity in the olfactory epithelium mediated by odorant exposure in zebrafish (Danio rerio) / Criastian Calfún Medina.

Por:

Calfún Medina, Cristian [author.]

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Tipo de material: Texto

TextoIdioma: Inglés Editor: Valparaíso, Chile : Universidad de Valparaíso, 2017Descripción: 139 hojasTema(s):

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Nota de disertación: Doctor en Ciencias con mención en Neurociencias. Resumen: We have previously shown that exposure to phenyl ethyl alcohol (PEA) causes an increase in the expression of the transcription factor otx2 in the olfactory epithelium (OE) of juvenile zebrafish, and this change is correlated with the formation of an odor memory of PEA. Here, we show that the changes in otx2 expression are specific to βPEA: exposure to αPEA did not affect otx2 expression. We identified 34 olfactory receptors (ORs) representing 16 families on 4 different chromosomes as candidates for direct regulation of OR expression via Otx2. Subsequent in silico analysis uncovered Hnf3b binding sites closely associated with Otx2 binding sites in the regions flanking the ORs. Analysis by quantitative polymerase chain reaction and RNA-seq of OR expression in developing zebrafish exposed to different isomers of PEA showed that a subset of ORs containing both Otx2/Hnf3b binding sites were down regulated only in βPEA-exposed juveniles and this change persisted through adult life. Localization of OR expression by in situ hybridization indicates the down regulation occurs at the level of RNA and not the number of cells expressing a given receptor. Finally, analysis of immediate early gene expression in the OE did not reveal changes in c-fos expression in response to either αPEA or βPEA.

Existencias ( 1 )
Notas de título ( 2 )

Existencias
Tipo de ítem	Biblioteca actual	Colección	Signatura topográfica	Estado	Fecha de vencimiento	Código de barras	Reserva de ítems
Tesis Postgrado	Ciencias Tesis	Tesis	M C151g 2017	Disponible		00405952

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Doctor en Ciencias con mención en Neurociencias.

We have previously shown that exposure to phenyl ethyl alcohol (PEA) causes an increase in the expression of the transcription factor otx2 in the olfactory epithelium (OE) of juvenile zebrafish, and this change is correlated with the formation of an odor memory of PEA. Here, we show that the changes in otx2 expression are specific to βPEA: exposure to αPEA did not affect otx2 expression. We identified 34 olfactory receptors (ORs) representing 16 families on 4 different chromosomes as candidates for direct regulation of OR expression via Otx2. Subsequent in silico analysis uncovered Hnf3b binding sites closely associated with Otx2 binding sites in the regions flanking the ORs. Analysis by quantitative polymerase chain reaction and RNA-seq of OR expression in developing zebrafish exposed to different isomers of PEA showed that a subset of ORs containing both Otx2/Hnf3b binding sites were down regulated only in βPEA-exposed juveniles and this change persisted through adult life. Localization of OR expression by in situ hybridization indicates the down regulation occurs at the level of RNA and not the number of cells expressing a given receptor. Finally, analysis of immediate early gene expression in the OE did not reveal changes in c-fos expression in response to either αPEA or βPEA.