000			02984nam a22002897i 4500
003			UVAL
005			20240507115358.0
007			t||
008			180711s2018 ||||| |||| 00| 0 eng d
040			_beng _cUVAL _aDIBRA _erda
084			_aM
100	1		_9224868 _aPiñeiro Feick, Miguel, _eauthor.
245	1	0	_aStudy of the role of CCAP neurons of the ecdysial behavorial sequence through mathematical and computational methods / _cMiguel Piñeiro Feick.
264		1	_aValparaíso : _bUniversidad de Valparaíso, _c2018.
300			_a67 hojas.
336			_atext _btxt _2rdacontent
337			_aunmediated _bn _2rdamedia
338			_avolume _bnc _2rdacarrier
502			_aDoctor en Ciencias mención en Neurociencias.
520			_aAnimal behavior arises from the interplay between neural, biomechanical and environmental dynamics. Neuromodulators, such as neuropeptides can regulate and reconfigure neural circuits to alter their output, affecting in this way animal behavior. How neuromodulators act on neural circuits to produce distinct behaviors is still poorly understood. Here I studied the peptidergic circuits that orchestrates the ecdysis behavioral sequence in Drosophila. This behavioral sequence has three phases: pre-ecdysis, ecdysis and post-ecdysis. The sequence is initiated by the release of ecdysis-triggering hormone (ETH) which activates multiple neuronal targets of which crustacean cardioactive peptide (CCAP) neurons have been identified as key for the successful execution of the behavioral sequence. How CCAP neurons regulate the behavioral sequence is still not clear, but studies of Manduca have showed that CCAP can initiate the ecdysial motor activity. I used calcium imaging data (from CCAP neurons only, and from CCAP neurons together with motoneurons), behavioral data from pupal ecdysis, and mathematical and computational approaches to study the dynamics and functional connectivity between CCAP neurons and motoneurons, and how they relate to the behavior. I found that CCAP neurons tightly regulate the motoneuronal activity during the ecdysis and post-ecdysis phases, consistent with the recent identification of CCAP targets. The behavioral analysis showed that while the three major phases of the ecdysis sequence can be detected in the motoneuronal recordings, motoneurons have major differences in the timing and regularity of their activity. Moreover, I found that the post-ecdysis phase has two subphases which appear to be absent in the motoneuro nal recordings. These results give us new insights into the peptidergic regulation of motor programs and behaviors.
650		0	_9161816 _aNEUROCIENCIAS COMPUTACIONAL.
650		4	_aMETODOS ESTADISTICOS _98298.
650		4	_aCONDUCTA ANIMAL _934263.
700	1		_aOrio, Patricio, _eDirector de tesis _9167655.
710	2		_aUniversidad de Valparaíso (Chile). _bFacultad de Ciencias. _bPrograma de Doctorado en Ciencias con mención en Neurociencias. _9234487.
942			_2ddc _c5
999			_c102912 _d102912